HPLC测定不同采收期水黄花中东莨菪内酯、金丝桃苷及槲皮苷含量

摘要

[ Abstract]Objective:To set up a method for simultaneous content determining of scopoletin,hyper-oside,quercetin in Euphorbia chrysocoma,and provide a reference basis to evaluate the best harvest period of the herb. Methods:Contents of scopoletin,hyperoside,quercetin in 22 batches of Euphorbi-a chrysocoma from Guiyang collected at different time points were tested by using HPLC. The column used was Phenomenon Luna C18(4. 6 mm × 250 mm,5μm),column temperature 40℃,flow speed 1. 0 mL/min,determining wave length 360 nm,and the mobile phase acetonitrile-0. 02% phosphoric acid. Gradient elution was conducted. Result:Good linear relationships of scopoletin,hyperoside and quercetin was showed within the ranges of 2 . 54~254 . 0 mg/L( r=0 . 999 3 ),0 . 67~67 . 0 mg/L( r﹥0. 999 5)and 1. 10 ~110 mg/L(r﹥0. 999 5)respectively. The recovery rate of 3 measured com-pounds were in the range of 98 . 72% and 100 . 0%,and each RSD was under 3%( n=6 ). The con-tents of 3 measured compounds showed large differences in 22 batches of determined herbs. Conclusion:Contents of the 3 kinds of compounds relate to the collection seasons. Herbs gathered during September through November have higher levels of the 3 compounds.%目的：建立水黄花中东莨菪内酯、金丝桃苷、槲皮苷的含量测定方法，为评价水黄花的最佳采收期提供依据。方法：采用高效液相色谱法对不同时间点采自贵阳市郊区的22批水黄花中的东莨菪碱、金丝桃苷及槲皮苷进行检测，色谱柱Phenomenon Luna C18柱（4.6 mm ×250 mm，5μm），柱温为40℃，流速为1.0 mL/min，检测波长为360 nm，流动相为乙腈-0.2%磷酸水，梯度洗脱；测定22批不同采收期的水黄花中3种成分的含量。结果：东莨菪内酯在2.54~254.0 mg/L（r＝0.9993）、金丝桃苷在0.67~67.0 mg/L（r＝0.9995）、槲皮苷在1.10~110 mg/L（r＝0.9995）的进样浓度范围内线性关系良好，加样回收率为98.72%~100.0%，RSD小于3%，22批药材中3种被测成分含量差距较大，9~11月采集的水黄花中3种成分含量较高。结论：建立了水黄花药材中3种主要成分东莨菪碱、金丝桃苷及槲皮苷含量测定方法，9~11月采集的水黄花中的3种主要成分含量较高。