硫辛酸注射液联合西洛他唑治疗糖尿病足的疗效及对氧化应激和炎症因子的影响

摘要

Objective:To investigate the effect of thioctic acid injection combined with cilostazol on the treatment of diabetic foot and its influence on oxidative stress and inflammatory cytokines.Methods:80 patients with diabetic foot treated in our hospital from February 2014 to February 2016 were selected and randomly divided into an experimental group (n=40) and a control group (n=39).All patients were given routine treatment.The patients in the control group received cilostazol,and those in the experimental group received thioctic acid injection combined with cilostazol.The clinical efficacy was observed.The levels of malondialdehyde (MDA),superoxide dismutase (SOD),advanced oxidation protein products (AOPP),interleukin (IL)-6,tumor necrosis factor α (TNF-α) and C-reactive protein (CRP) in serum were measured.The protein expression of S100A12 was detected by western blotting.Results:The response rate in the observation group (92.5％) was significantly higher than that in the control group (71.8％) (P＜0.05).Before treatment,there were no significant differences in above indexes between the two groups (P＞0.05).After four weeks of treatment,the serum levels of MDA,AOPP,IL-6,TNF-α and CRP as well as the protein expression of S100A12 in the experimental group were significantly lower than those in the control group,while SOD level was significantly higher (P＜0.05).There were no significant differences in the incidence of adverse reactions (P＞0.05).Conclusion:Thioctic acid injection combined with cilostazol in the treatment of diabetic foot was remarkably effective.This combination could inhibit oxidative stress and inflammation,and was safety.%目的:探讨硫辛酸注射液联合西洛他唑治疗糖尿病足(DF)的疗效及对氧化应激、炎症因子的影响.方法:选取2014年2月至2016年2月陕西省安康市人民医院收治的79例DF患者,随机分为研究组(n=40)和对照组(n=39).两组均给予常规治疗,对照组在此基础上给予西洛他唑治疗,研究组给予硫辛酸注射液联合西洛他唑治疗.比较两组患者的临床疗效和不良反应发生率,观察两组治疗前、后血清中氧化应激指标[丙二醛(MDA)、过氧化物歧化酶(SOD)、晚期蛋白氧化产物(AOPP)]和炎症因子[白细胞介素6(IL-6)、肿瘤坏死因子-α(TNF-α)及C反应蛋白(CRP)]的水平,同时采用western blotting法检测外周血中S100A12蛋白的表达.结果:研究组的治疗总有效率为92.5％,显著高于对照组的71.8％(P＜0.05).治疗前,两组患者上述各指标比较,差异均无统计学意义(均P＞0.05);治疗4周后,与对照组比较,研究组患者血清MDA、AOPP、IL-6、TNF-α、CRP水平及外周血中S100A12蛋白表达水甲显著降低(P＜0.05),SOD含量显著升高(P＜0.05).两组不良反应发生率比较,差异无统计学意义(P＞0.05).结论:硫辛酸注射液联合西洛他唑治疗DF的效果显著,可明显改善氧化应激状态,抑制炎症反应,且安全性较好.