秀珍菇废渣中木质素降解酶系的研究

摘要

【目的】重点研究秀珍菇废渣中的木质素降解酶系。【方法】对秀珍菇废渣中可能存在的3种木质素降解酶：锰过氧化物酶（Manganese peroxidase，MnP）、木质素过氧化物酶（Lignin peroxidase，LiP）及漆酶（Laccase， Lac）进行活力测定，并对漆酶的最适 pH值和最适温度进行考察。【结果】在秀珍菇废渣中未检测到锰过氧化物酶活力，检测到漆酶和木质素过氧化物酶活力。漆酶的最适 pH 值为6．2，最适温度为30℃。【结论】较一般食用菌不同，秀珍菇废渣中同时具有木质素过氧化物酶和漆酶活力，并且木质素过氧化物酶活力还较高。%Objective This paper focuses on lignin degrading enzymes of pleurotus geestera-nus.[Methods]Manganese peroxidase,lignin peroxidase and laccase from pleurotus geestera-nus were detected.Optimal pH and temperature of laccase were also analyzed.[Results]The results showed that only manganese peroxidase and laccase were assayed in the pleurotus geesteranus residues and no lignin peroxidase were checked.Optimal pH and temperature of laccase were 6.2 and 30℃,respectively.[Conclusion]Different from normal mushroom,pleu-rotus geesteranus showed both lignin peroxidase and laccase activity.