薄层色谱法评价不同产地黄根的品质

摘要

【目的】建立评价黄根(Prismatomeris connate Y.Z.Ruan)品质的薄层色谱分析方法。【方法】以有效成分甲基异茜草素为指标，以甲苯-乙酸乙酯-甲醇-甲酸(3：1：0.2：0.1)为展开剂，建立黄根的专属性薄层色谱，并评价10个不同产地黄根的品质。【结果】不同产地黄根药材与对照色谱在相应位置上，显示出相同颜色的斑点，且斑点清晰，比移值(Rf)为0.76。【结论】薄层色谱法能够有效对黄根进行鉴别，且该方法简便、准确，具有较好的适应性。%Objective]To establish a TLC method for the identification of Prismatomeris connate Y.Z.Ruan.[Methods]Rubiadin was indicators,methylbenzene-ethyl acetate-metha-nol-formic acid (3:1:0.2:0.1)was developing agent.[Results]The same color spots were shown in the corresponding position of the Prismatomeris connate Y.Z.Ruan from different origin.The spots were clear and the Rf index was 0.76.[Conclusion]The TLC method is ef-fective for the identification of the Prismatomeris connate Y.Z.Ruan.The method is simple and reliable.