通过半定量反转录RT—PCR的方法，研究了不同配比的复合材料纳米羟基磷灰石／聚已内酯／（nHA／PCL）（nHA：PCL1=40：60和nHA：PCL2=60：40）对成骨细胞骨相关基因（成骨细胞碱性磷酸酶、骨钙素蛋白、骨粘连蛋白和骨桥蛋白等）表达的影响．结果表明：在nHA／PCL复合材料上生长的成骨细胞的骨钙蛋白、骨粘连蛋白和骨桥蛋白mRNA表达量上调；在PCL材料上生长的成骨细胞的骨钙蛋白、骨粘连蛋白和骨桥蛋白mRNA的表达量下调；而nHA／PCL和PCL均使碱性磷酸酶基因的表达下调。%To study the effects of nano--hydroxyapatite/polycaprolaetone（nHA/PCL）composites on bone-- related genes expression in osteoblasts in vitro,3 different proportions of nHA/PCL （nHA：PCL1 = 0：100, nHA： PCL2 = 40：60 and nHA· PCL2= 60： 40） were prepared to treat cultured osteblasts. Reverse transcrip- tion PCR assay was used to analyze the expression of bone--related genes （alkaline phosphatase, osteocalcin, osteonectin and 0steopontin）. The results showed that the mRNA expression of osteocalcin,osteonectin and osteopontin in cultured osteoblasts grown on the nHA/PCL composites （nHA.PCL=4O-60 and 60：40） were higher than the control; while those in osteoblasts on PCL（nHA：PCL1=0：100）were lower than the control. The expression alkaline phosphatase was a little inhibited by both nHA/PCL and PCL.
展开▼
