再生细菌纤维素膜的制备与性能表征

摘要

In this work,regenerated bacterial cellulose (RBC)membrane was prepared by LiCl/DMAc,and in-fluence of the concentration of bacterial cellulose solution on crystallinity,equilibrium water content,light transmittance and the diffusion of nutrients (sodium chloride,glucose sugar,tryptophan)of RBC membrane were studied.The experimental results showed that,with the concentration of bacterial cellulose solution in-creasing,the degree of crystallinity of regenerated membrane slightly decreased;equilibrium water content in-creased slightly and light transmittance of dry films changed little,but that of wet films changed obviously;the diffusion coefficient to nutrients hardly changed with the BC solution concentration,and that to glucose sugar and tryptophan were approximately 1.0 × 10-6 cm2/s,and the sodium chloride permeability coefficient was about 2.5×10-6 cm2/s.This work indicated that RBC membrane was promising biomaterial for corneal lamellar transplantation application.%采用LiCl/DMAc的溶解体系将细菌纤维素溶解后制备再生细菌纤维素(RBC)膜,并研究了成膜的细菌纤维素溶液浓度对膜的结晶度、平衡含水率、透光率以及对营养物质(氯化钠、葡萄糖、色氨酸)的透过性能的影响.实验结果表明,随着成膜溶液浓度的升高,再生膜的结晶度有一定程度的降低；平衡含水率稍有升高；再生膜干态下的透光率变化不大,但浸泡PBS后膜变化较大；对营养物质的透过系数随浓度变化较小,对葡萄糖和色氨酸的透过系数相近,约为1．0×10-6 cm2/s,氯化钠的透过率系数约为2．5×10-6 cm2/s.研究表明再生细菌纤维素膜是一种潜在的人工角膜板层移植材料.