基于上转换荧光共振能量转移检测∗

摘要

设计了一种基于分子信标的新型生物检测模型，以上转化发光纳米粒子(UCPs)作为 FRET 能量供体，利用分子信标茎环结构的优势，有效减小供受体之间距离，从而增加 FRET 能量转移效率，提高猝灭效率和生物检测灵敏度，并将该模型用于目标 DNA和凝血酶检测，得到了令人满意的结果。此外，将这种灵敏且结构简单的模型用于复杂基质中凝血酶的检测，显示出 UCPs 优越的抗干扰性能。%In this study,we design a new biosensor using up-converting phosphors (UCPs)as the energy donor for biomolecules sensing.The problem of the limited quenching efficiency was effectively resolved by construc-ting MB sensors in this work.Because of the stem-loop hairpin structure,it can reduce the donor-to-acceptor distance and hence significantly increased the energy transfer efficiency and improved the sensitivity.We apply this sensor in the detection of target-DNA and thrombin with satisfactory results obtained.What’s more,the up-converting MB sensor in simple and flexible structure was used to monitor thrombin in complicated biological sample matrix,demonstrating the ability of anti-interference.