基于上转换荧光纳米粒子和金纳米粒子间荧光共振能量转移的高灵敏赭曲霉毒素A检测方法研究

摘要

Upconversion nanoparticles were synthesized and modified with OTA aptamer,and gold nanoparticles were modified with complementary DNA strand(cDNA).Based these nanoparticles,a FRET aptasensor for OTA was successfully fabricated by using upconversion nanoparticles as energy donor and gold nanoparticles as energy acceptor.In the optimum conditions,this aptasensor obtained a detection limit of 0.001 ng/mL with a linear range of 0.001-10 ng/mL.The above method was applied in the detection of OTA in beer samples.At spiked levels of 0.01,0.1,1.0 ng/mL,the recoveries for OTA ranged from 100％ to 119％ with the relative standard deviations(RSDs) of 4.3％-4.9％.This method also demonstrated its advantages such as high sensitivity,good specificity,simplicity and low cost.%制备了水溶性的上转换荧光纳米材料,在其表面修饰赭曲霉毒素A(OTA)适配体作为能量供体探针;在金纳米粒子表面修饰OTA适配体互补链作为能量受体探针,构建了OTA适配体传感器.在最优条件下,OTA的检测范围为0.001 ～ 10 ng/mL,检出限可达0.001 ng/mL.将其应用于啤酒样品中OTA的检测,当加标水平为0.01、0.1、1.0 ng/mL时,回收率为100％～ 119％,相对标准偏差为4.3％ ～4.9％,表明该方法可用于实际样品检测.该方法具有灵敏度高、特异性好、操作简单、成本较低等优点.