Objective To investigate the effects of Koumine on spontaneous pain of peripheral nerve injury-induced neuropathic pain . Methods Rat chronic constriction injury (CCI) model of neuro-pathic pain was used to observe the effects of Koumine administered subcutaneously one time at dose of 7 .0 ,1 .4 or 0 .28 mg/kg ,or twice a day for 7 consecutive days at 7 .0 or 0 .28 mg/kg on spontaneous pain indicated by ipsilateral foot-lifting time and postural index . Results Single dose of Koumine reduced both foot-lifting time and the postural index in a dose-dependent manner ,in particular ,the difference in the foot-lifting time between 7 .0 mg/kg group and untreated control group was statistically significant (P<0 .01 ) whereas the significant difference in the postural index was observed in both 1 .4 and 7 .0 mg/kg group as compared with the control group (P<0 .05 and P<0 .01 respectively ) . Repeated administration of 7 .0 mg/kg Koumine shortened spontaneous foot-lifting time at days 3 ,5 ,7 and 9 post CCI (P<0 .01) . Similarly ,Koumine repeated dosing at 7 .0 mg/kg also ameliorated the postural index at days 3 ,5 ,7 and 9 after the injury (P<0 .05 or P<0 .01) with the therapeutic effect being most prominent at day 9 after the CCI operation . Conclusion Koumine has pain-relieving effect on spontaneous pain of peripheral nerve injury-induced neuropathic pain .%目的：观察钩吻素子抗外周神经损伤致神经病理性疼痛（NPP）中自发痛的效应。方法在大鼠坐骨神经慢性束缚损伤（CCI）NPP 模型上，以术侧自发性抬足时间和足姿势评分为指标，观察单次（7．0，1．4，0．28 mg/kg）、连续多次（7．0，0．28 mg/kg ，每日2次，连续7 d）皮下注射给予钩吻素子对 NPP中自发痛的效应。结果单次皮下注射给予钩吻素子剂量依赖性减少自发性抬足时间，其中7．0 mg/kg组与对照组比较差别有统计学意义（P＜0．01）；单次皮下注射给予钩吻素子剂量依赖性降低足姿势评分，其中1．4，7．0 mg/kg组与对照组比较差别有统计学意义（P＜0．05和 P＜0．01）。连续多次给予钩吻素子减少自发性抬足时间，7．0 mg/kg组在术后第3，5，7，9 d自发性抬足时间均低于对照组（P＜0．01）；连续多次给予钩吻素子降低足姿势评分，7．0 mg/kg组在术后第3，5，7，9 d评分均低于对照组（P＜0．05或 P＜0．01），到术后第9 d疗效最强。结论钩吻素子具有抗外周神经损伤致NPP中自发痛的效应。
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