DNA定量技术在死亡时间推断中的研究进展

摘要

Estimation of postmortem interval (PMI) is one of the difficult problems in forensic medicine.With the development of molecular biological techniques, DNA quantification methods were widely applied in estimating PMI. The postmortem degradation of DNA in different tissues and organs was discussed in this article and the recent DNA quantitative techniques being used for estimating PMI were reviewed.These techniques included single cell gel electrophoresis, Feulgen staining image analysis, flow cytometry.%死亡时间推断是法医病理学研究的热点及难点之一.随着分子生物学技术的发展,DNA的定量已广泛应用于死亡时间推断的研究.本文主要对机体死后不同组织和器官中DNA含量随时间的降解情况,以及单细胞凝胶电泳、Feulgen染色图像分析技术、流式细胞仪等近年来新的DNA定量技术应用于死亡时间推断研究中的进展进行了综述.