大鼠原发性脑干损伤后 S100B 和 GFAP 的表达变化

摘要

目的：观察大鼠原发性脑干损伤后脑干组织中 S100B、胶质纤维酸性蛋白（glial fibrillory acidic protein，GFAP）的表达变化，探讨其与脑干损伤时间的变化规律及在损伤中的作用机制。方法建立机械性打击脑干损伤动物模型，利用 HE 染色、Gless 嗜银染色和 SP 免疫组织化学法观察脑干组织中 S100B、GFAP 在损伤后不同时间的表达，并应用图像分析技术对其进行统计学分析。结果 S100B 阳性表达于伤后30 min 开始增多，随时间延长逐渐升高，于24 h 达到高峰后开始下降，并于72 h 基本降至正常水平；GFAP 阳性表达于伤后30 min 开始升高，48 h 达到高峰后开始下降，但仍高于对照组。结论原发性脑干损伤后 S100B、GFAP 的表达具有时间规律性，在原发性脑干损伤时间的推断及神经修复过程中具有一定的作用。%Objective To study the expression of S100B and glial fibrillory acidic protein (GFAP) after primary brainstem injury in rat and discuss the changes with brainstern injury time and their mechanism in the injury. Methods The brainstem injury animal model was established using the mechanical impact-ing method. The HE staining, Gless argentaffin staining and SP immunohistochemical method were ap-plied to observe the changes of S100B and GFAP at different injury time. The immunostaining results were measured statistically with imaging analysis technology. Results A large number of S100B positive cells could be seen in 30 min. Afterward, expression increased gradually with time and peaked up in 24 h, and reversed back the normal in 72 h. The GFAP positive cells showed rise continually in 30 min, and reached the peak in 48 h, then started to decrease, but still higher than that in control. Conclusion The expression of S100B and GFAP is correlated with post traumatic intervals after brainstem injury in rat, and may be useful in estimation post traumatic intervals and nerve regeneration.