对酪蛋白-葡聚糖共聚物胶束作为输送体系而言,所包埋营养素的总量是评价其质量的重要指标.表面活性剂十二烷基硫酸钠(SDS)在将共聚物胶束解体的同时使辅酶Q10增溶于水中,从而便于进行定量分析.实验结果表明:当SDS浓度为0.31 mol/L、孵育温度30℃、硼氢化钠7mg/mL、用量100 μL、还原时间3～5 min时,SDS增溶法的加样回收率在(98.77士1.60)％～(95.90士0.80)％之间.同时,共聚物中酪蛋白单元的内源荧光和外源荧光光谱分析显示,当SDS浓度为0～0.5 mol/L时,SDS会部分改变酪蛋白单元的疏水结构；且SDS浓度为0.31 mol/L时,SDS与酪蛋白单元疏水区的相互作用几乎不受温度的影响.%The total content of nutrient is an important index for evaluating the quality of casein-dextran copolymer micellers as a delivery system. Sodium dodecyl sulfate (SDS) as a surfactant can be used to disorganize copolymer micellers and make coenzyme Q10 solubilize in water for quantitative analysis. In this study, the recovery rate of coenzyme Q10 from blank casein-dextran copolymer micellers ranged from (98. 77 ±1.60) % to (95. 90 ±0.80) % with conditions as follows; SDS concentration 0.31 mol/L, incubation temperature 30 °C, sodium borohydride (7 mg/mL) dL, reduction time 3~5 min. The analysis of synchronous and exogenous fluorescence spectra indicated that the hydrophobic region of casein unit of copolymer was partly changed during solubilization at SDS concentration form 0 to 0. 5 mol/L. The interaction between SDS and hydrophobic region was not influenced by temperature when the concentration of SDS was 0. 31 mol/L.
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