秀珍菇子实体多糖的提取工艺优化及体外抗氧化性

摘要

采用响应面法优化了秀珍菇子实体中水溶性多糖的超声提取工艺,并初步测定了多糖提取物的抗氧化活性.结果表明,在提取温度为40℃、超声功率为400W、料液比为1∶30、浸提时间114 min的最佳提取工艺条件下,响应面拟和所得方程对秀珍菇多糖的最大提取率预测值60.74mg/g,实测值60.19 mg/g,实测结果与预测值符合良好.秀珍菇的多糖提取物具有清除羟基自由基的作用,在一定范围内随着多糖浓度的增加其清除效果度还原力均加强.%The ultrasonic extraction process of polysaccharides from Pleurotus geesteranus was optimized by response surface analysis,and the antioxidant activity of the extracts were determined. The results showed that,the optimum ultrasonic extraction conditions were obtained as follows; temperature at 40 ^.ultrasonic power at 400 W,time duration for 114 min,and the ratio of solution (water) to solid (fruit body powder) of 30:1. Under this condition,the extraction yield of the polysaccharide from Pleurotus geesteranus was 60.19 mg/g,which was consistent with the predicted value (60.74 mg/g). The Polysaccharide extracts have a certain extent function to scavenging hydroxyl radical. Their antioxidant power and the reducing capacity increased with the increase of polysaccharides concentration.