液态发酵茯苓菌丝体形态与产胞内三萜的关系

摘要

This research aims at investigating the effects of fermentation conditions on mycelium morphology and total triterpenoids yield during submerged culture of Poria cocos.The results indicated that the total triterpenoids main derived from middle-sized equivalent diameter and pellet in smooth shape were more conducive to enhancing the total triterpenoids production.The appropriate condition of submerged culture were broth content 50 mL,inoculate size 6％,pH 5.5,temperature 26,℃,rotation speed 130 r/min for 11 days.The positive correlations both production of total triterpenoids and M diameter growth as well as smooth pellet have shown that the relationship between mycelium morphology and intracellular triterpenoids.%研究茯苓菌丝体液态培养条件对菌丝体形态及胞内三萜产量的影响.结果表明,当装液量为50 mL,接种体积分数6％,pH 5.5,温度26℃,转速130 r/min,液态培养11d时,有利于中型和光滑型菌球的生长及胞内总三萜的积累,且中型和光滑型菌球的生长与胞内总三萜的积累量呈正相关,说明茯苓液态发酵过程中菌丝体形态与胞内三萜积累量相关联.