超声波法提取茯苓菌丝体胞内多糖的研究

摘要

In order to better use intracellular polysaccharide from Poria cocos mycelia ,the optimal extraction technology of intracellular polysaccharide from Poria cocos mycelia by ultrasonic wave was investigated .Based on the single factor design ,the extraction technology of intracellular polysaccharide was optimized by the re‐sponse surface methodology .The results showed that the optimal extraction technology were as follows :liquid and solid ratio of 23∶1 ,ultrasonic power 100 W ,extraction temperature 73℃ and extraction time 27 min .Under those conditions ,the extraction rate of polysaccharide was 2 .99% .%　　为了更好地利用茯苓菌丝体胞内多糖，对茯苓菌丝体胞内多糖的超声波法提取工艺进行了研究。在单因素试验的基础上，采用响应面法试验，优化了超声波法提取工艺。结果表明：优化的胞内多糖超声波法提取工艺条件为液料比23∶1，超声功率100 W ，提取温度66℃，提取时间27 min。在此条件下，多糖提取率为2．99％。