基于HTS-ELISA的单克隆抗体分泌杂交瘤细胞筛选技术进展

摘要

Various immune analysis technologies based on the monoclonal antibody are the main develop-ment direction for modern food safety rapid detection technology. Therefore, preparation of monoclonal anti-bodies with high-activity has got more and more attention by researchers. Preparation of monoclonal antibody usually involves many techniques, including the preparation of the antigen, immune design, cell fusion, screening of active hybridoma and antibody purification. Among them, the screening of monoclonal antibody secreting hybridoma cells is a key of the prepared monoclonal antibody part. Therefore, in order to provide a reference for high-activity monoclonal antibody preparation and the development of rapid detection technology for food safety, this paper focused on low density fusion cell culture, special equipment and the principle and technology of determining hybridoma cell activity in the screening process according to drag the number of holes (Trailing) to related activities, and mainly introduced one kind of high-activity monoclonal antibody se-cretion hybridoma technique which was based on HTS-ELISA screening method.%基于单克隆抗体的各类免疫分析技术是现代食品安全快速检测技术发展的主要方向之一，因此，高活性单克隆抗体的制备方法自然为众多研究者所关注。通常单克隆抗体的制备过程涉及到很多技术环节，包括抗原制备、免疫剂量设计、细胞融合、活性杂交瘤细胞筛选以及抗体纯化等，其中分泌单克隆抗体杂交瘤细胞的筛选环节是单克隆抗体制备的关键一环。为此，本文主要介绍了一种基于HTS-ELISA的高活性单克隆抗体分泌杂交瘤细胞的筛选技术，重点是对融合细胞的低密度培养、特殊仪器设备和在筛选过程中如何依据拖孔数(Trailing)来判断杂交瘤细胞的活性等相关的原理和技术，旨在为我国高活性单克隆抗体制备技术的发展及加快食品安全快速检测技术的发展提供一个参考方法。