Hybridoma Cell Growth and Monoclonal Antibody Yield in Serum-Free Media

摘要

This study compares the relative capacities of four serum-free media to supportgrowth and MAb production for three mouse hybridoma cell lines. A 10% foetal bovine serum-Iscove's medium served as the control medium. The four serum-free media included Ventrex HL-1, Iscove's with 2% Ultroser HY, HybriMax DME/F12, and KC 2000. Ultroser HY supported the best cell growth of the serum-free media. The greatest antibody yields for hybridoma TDIII, IIIBB2 were observed with Ultroser HY and KC 2000 whereas hybridoma TDII, IAA11 secreted the most MAb in Ultroser HY but only 0-0.04mg/ml with the other serum-free media. Serum-free media were not suitable for MAb production by hybridoma BGII, VF9/2D. Serum-free media never supported growth levels and MAb yields as high as serum-supplemented media. MAb production and growth in serum-free media were variable and dependent on the specific hybridoma cell line cultured.