VIDAS在保健食品沙门氏菌快速检测中的应用

摘要

ABSTRACT:Objective The automated immunoassay analyzer (VIDAS) was applied to detect the Salmonella in health food.Methods The 21 lots of health food were divided into BPW group and BPW positive control group. TheSalmonella in 21 lots of health foods were detected by both VIDAS method and GB method. The results were compared.Results All the results were negative in BPW group. Results of both VIDAS method and GB method in 20 lots of health food were consistent with each other in BPW positive control group, and the percentage accounted for 95.2% of the total.Conclusion VIDAS method could be used to fast detect theSalmonella in health food. It took 96 h at least to detectSalmonella by GB method. However, it took only 48 h by VIDAS method. It could be the final result if the result was negative, and it should be confirmed according to the GB method if the result was positive.%目的：将全自动免疫分析仪(VIDAS)应用于保健食品的沙门氏菌快速检验。方法将21批次保健食品分为BPW组和BPW阳性对照组，分别采用VIDAS和国标方法进行沙门氏菌检验，并比较结果。结果21批保健食品的沙门氏菌检验中， BPW组两种检验方法结果均为阴性； BPW阳性对照组有20批的VIDAS方法与GB方法的检验结果一致，占总批数的95.2%。结论可以用VIDAS方法对保健食品进行沙门氏菌的快速检验。用国标方法检验沙门氏菌需要96 h或者更长，用VIDAS方法则可以把检验时间缩短到48 h。如果VIDAS方法检验结果为阴性则可以作为最终结果，如果结果为阳性，则需要按照国标方法进一步进行确认试验。