本研究建立中国健康成人外周血B淋巴细胞各种免疫标志的参考范围,为临床正确诊断B淋巴细胞增殖性疾病提供基础信息.采用全血溶RBC法和SSC/CD19设门技术以三色流式细胞术对来自41名健康成人EDTA-K2抗凝静脉血进行免疫表型分析.结果表明: 外周血B淋巴细胞几乎全部表达CD22,CD20,CD62L,CD40,CD24,CD79b,CD79a和FMC-7分子,几乎不表达CD11a,CD80,CD103,CD10,CD40L,CD54,CD95L,CD86和CD95分子,CD18,CD44,CD23,CD5,CD11c和CD43的阳性率各不相同,78%的B细胞为IgD阳性、κ/λ比例为1.26.结论:为了对患者的免疫分型资料进行准确判读和对B淋巴细胞增殖性疾病作出正确的诊断,必须考虑正常B细胞群体中各种标志的表达情况.同时,在临床工作中不宜直接套用国外的标准,而必须注意中国人和西方人群之间在某些B细胞标志方面可能存在的差异.%To establish reference values of various immunophenotypic markers in B lymphocyte population in healthy Chinese adults and build background information for accurate interpretation of B cell immunophenotyping data in clinical practice, peripheral blood from 41 healthy adults were collected separately into test tubes containing EDTA-K2 and stored in room temperature no more than 24 hours before analysis.Whole blood lysis technique and multiparameter flow cytometry were applied to immunophenotype B cells gated on CD19/SSC dot-plot. The results showed that CD22,CD20,CD62L,CD40,CD24,CD79b,CD79a, and FMC-7 were almost positive in the circulating B cell population,whereas CD11a,CD80,CD103,CD10,CD40L,CD54,CD95L,CD86,and CD95 were almost negative in the perapheral blood B lymphocytes.CD18,CD44,CD23,CD5,CD11c and CD43 were positive in different B cell subpopulations. 78% of B cells were IgD positive and ratio κ/λ was 1.26. The significance of all these markers in the differential diagnosis of lymphoproliferative diseases was discussed. The conclusion is that it is necessary to consider the qualitative and quantitative levels of expression of various markers in normal B cell population in order to accurately interpret the pathologcal immunophenotypic data in clinical practice. It is also important to note the immunotypic differences of B cells between Chinese and Western populations.
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