HLA-A、B、DRB1基因与白血病的相关性研究

摘要

本研究从白血病患者HLA基因的分布特征探讨其与白血病的相关性.采用序列特异性引物聚合酶链反应分型技术(PCR-SSP)对2994例健康新生儿脐带血进行HLA基因分型,与1246例在我库查询的白血病患者HLA基因分型进行了分析比较,探讨HLA基因分布频率在两组中的差异.结果表明,疾病组与对照组相比,HLA-B* 56(0.56％)、B*70(0.24％)基因频率明显增高(RR=2.2546、6.2598、x2 =5、5.98、P＜0.05)；HLA-A*03(3.45％)、A* 30(4.86％)、B* 13(8.75％)、B*44(3.25％)、B*61(5.70％)、DRB1* 07(8.23％)、DRB1* 15(14.21％)基因频率明显降低(RR=0.5889、0.7187、0.7359、0.5713、0.7127、0.6242、0.7976、x2=19.23、9.82、14.33、20.48、11.99、33.21、11.56、P＜0.01).结论:HLA-B*56、B*70基因有遗传易感性,为白血病发生的危险标志；HLA-A* 03、A*30、B*13、B*44、B*61、DRB1* 07、DRB1* 15基因是白血病的保护性标志.%This study was aimed to investigate the corelation between HLA gene distribution and allele frequency of the patients with leukemia. PCR-SSP technique was used to detect the HLA genotype of 2994 umbilical cord blood units from healthy newborns (as control) , the detecting result of which was compared with HLA genotypes of 1246 patients with leukemia searched in our cord blood bank. The differences between two groups were compared and analyzed. The results indicated that as compared with the control group, the allele frequencies of HLA-B * 56(0. 56%) 、5 * 70 (0. 24% ) obviously increased(RR =2.2546、6.2598 、χ2 =5 、5.98、P <0.05), while the allele frequencies of HLA -A * 03(3.45%),、A* 30(4. 86%)、B*13(8.75%) 、B44 * (3. 25%),、B61 * (5. 70% )、DRB1 * 07(8. 23% ) 、DRB1 * 15 (14.21% ) obviously decreased in patients with leukemia ( RR =0. 5889、0. 7187、0. 7359、0. 5713、0. 7127、0. 6242、 0.7976、χ2=19.23、9. 82、14. 33、20. 48、11. 99、33. 21、11.56,P<0.01) . It is concluded that HLA -B * 56、B * 70 alleles seem to be characterized by the genetic susceptibility to leukemia and may be served as risk markers for leukemia occurence, while the HLA-A * 03 、A * 30、B * 13、B * 44、B * 61、DRB1 * 07、DRB1 * 15 can be consideved as genetic indicators for resistance of leukemia.