IGF-IR和IR在儿童急性淋巴细胞白血病中的表达

摘要

This study was aimed to investigate the mRNA expression levels of insulin-like growth factor- I receptor (IGF- IR) and insulin receptor (IR) in peripheral blood lymphocytes of childhood ALL and their relationship with clinical features. The expression levels of IR and IGF- IR in peripheral blood lymphocytes of ALL children and healthy children were analysed. Peripheral blood samples were obtained from 43 ALL patients and 14 healthy children. The mRNA expression of IGF- IR and IR was measured by RT-PCR. The results showed that IGF- IR was widely expressed on peripheral blood lymphocytes of ALL children and healthy controls, mRNA level of IGF- IR significantly increased in ALL children of newly diagnosed, relapsed and hyperglycemia groups (P = 0. 000, P = 0.002 and P = 0.05) as compared with the normal control group. mRNA level of IGF- IR in CR group was significantly lower than that in newly diagnosed and relapsed groups (P = 0. 000 and P = 0.018); mRNA level of IR in newly diagnosed and relapsd groups were significantly lower than that in the normal control group (P = 0. 001 and P = 0. 018 ). mRNA level of IR in CR group was significantly higher than that in newly diagnosed and relapsed groups (P =0.000 and P =0. 001) ; mRNA levels of IGF- IR and IR correlated with the clinical stages of the disease, while they had no association with the sex, age and white blood cell counts at new diagnosis. It is concluded that IGF- IR positively correlates with the stages of the disease, indicating that IGF- IR plays a leading role in the proliferation of tumor cells, and its increased expression levels may reflect the malignant proliferation of ALL cells.%本研究的目的是探讨胰岛素样生长因子Ⅰ受体(insulin-like growth factor-Ⅰ receptor,IGF-IR)、胰岛素受体(insulin receptor,IR)在儿童急性淋巴细胞白血病(ALL)患者的外周血淋巴细胞表面的表达及其与临床特征的关系.收集43例儿童ALL和14例健康儿童外周血标本,采用实时定量PCR检测IGF-IR和IR mRNA的表达.结果表明:儿童ALL和正常儿童的外周血淋巴细胞表面均表达IGF-IR mRNA.ALL初发组、复发组及高血糖组的IGF-IR mRNA表达水平与正常对照组相比明显升高(P=0.000,P=0.002和P=0.05).完全缓解组与初发组和复发组相比,IGF-IR mRNA表达水平明显减低(P=0.000和P=0.018).初发组和复发组ALL患儿外周血淋巴细胞IR mRNA表达水平明显低于正常对照组(P=0.001和P=0.018).完全缓解组与初发组和复发组相比表达量升高,差异有统计学意义(P=0.000和P=0.001).ALL儿童外周血淋巴细胞IGF-IR mRNA和IR mRNA在不同病程阶段的表达差异有统计学意义,但表达水平与性别、年龄及初诊时白血病计数无关.结论:IGF-IR的表达与儿童ALL病程相关,病情进展时(初发、复发)表达升高,病情缓解(CR)时则降低,提示IGF-IR在ALL增殖中起重要作用,其表达量增高可能反映了ALL细胞恶性增殖倾向.