antibody that binds to igf-ir, pharmaceutical composition and method of manufacture, hybridoma cell lines, nucleic acid, expression vector, host cell, 5 and methods for producing a polypeptide that binds to igf-ir and inhibits the binding of igf-i and igf-ii to igf-ir, and for the selection of an anti-igf-ir antibody from a plurality of anti-igf-ir antibodies

摘要

"antibodies to insulin-like growth factor i receptor and uses thereof". the present invention relates to an antibody binding to igf-ir and inhibiting the binding of igf-i and igf-ii to igf-ir which is characterized in that said antibody is a) is of igg1 isotype, b) shows a ratio of ic ~ 50 ~ values for inhibiting the binding of igf-i to igf-ir to inhibiting the binding of igf-ii to igf-ir from 1: 3 to 3: 1, c) inhibits at least 80 % at a 5 nm concentration of igf-ir phosphorylation in a cell phosphorylation assay using 3t3 cells providing 400,000 to 600,000 igf-ir molecules per cell in a medium containing 0.5% heat-inactivated fetal calf serum (fcs) when compared to such an assay without said antibody, d) does not show any stimulating activity of igf-ir measured as phosphorylation of igf-ir at a concentration of 10 109 m in a cell phosphorylation assay using cells from 3t3 supplying 400,000 to 600,000 igf-ir molecules per cell in a medium containing 0.5% inactive fetal calf serum heat-treated (fcs) when compared to such an assay without said antibody has improved properties in antitumor therapy.