This study was aimed to investigate the factors influencing mobilization efficiency of peripheral hematopoietic stem cells with granulocyte colony stimulating factor (G-CSF) and their impact on healthy donors. 181 donors were mobilized subcutaneously with G-CSF at 5 - 10 μg/(kg·d), and 10 donors were mobilized with G-CSF at 3. 3 -4. 9 μg/(kg·d), once 12 h, for 4 -5 d. Peripheral blood mononuclear cell (MNC) and CD34+ cell counts were analyzed by flow cytometry. Mobilization-related side effects were also monitored. The results showed that white blood cell counts increased by 6 times averagely after mobilization (P < 0. 01). The platelet count obviously decreased ( P < 0. 01), while the hemoglobin level did not show significant difference. No significant differences were observed in MNC and CD34+ cell counts between those subjects harvested on the 4th and 5th day after mobilization. Male donors were superior to female ones in cell harvest( P < 0.01). Donor body weight played positive role in cell yield, while impact of age on harvest was not remarkable. Neither MNC nor CD34+ cell count showed a linear relationship with G-CSF dose. Only slight side effects were observed on the donors in this study. It is concluded that mobilization with G-CSF is sufficient in healthy donors without remarkable side effects.%本研究旨在探讨粒细胞集落刺激因子(G-CSF)动员外周血造血干细胞的影响因素及对健康供者的影响.181例志愿健康供者应用G-CSF 5-10 μg/(kg·d)动员,10例应用G-CSF 3.3-4.9μg/(kg.d)动员,12h 1次,连续动员4-5 d；采集、检测外周血中单个核细胞(MNC)数与CD34+细胞数,并观察供者动员及采集过程中的不良反应.结果表明,与动员前相比,动员后(采集前)供者外周血白细胞数平均升高7倍(P＜0.01)；血小板数明显下降(P＜0.01)；血红蛋白含量无明显差异性.动员第4或5天采集效果无差异.男性供者采集的MNC、CD34+细胞数高于女性(P＜0.01),高体重供者采集的MNC、CD34+数高于低体重供者,年龄对采集效果无明显影响.G-CSF剂量与采集效果无线性关系.供者不良反应轻微.结论:G-CSF可以有效动员外周血造血干细胞,供者无明显的不良反应.
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