Livin 基因在结直肠癌中的表达及其与 PTEN 和Caspase-3的相关性研究

摘要

Objective To explore Livin,PTEN and Caspase-3 protein expression and their correlationship in colon canc-er.Methods The expressions of Livin ,PTEN and Caspase-3 protein in 50 cases of colon cancer , colon adenoma and nor-mal colorectal mucosa were detectded by immunohistochemistry staining .Results (1)The expression of Livin in colon canc-er, colon adenoma and normal colorectal mucosa was 64.00%,56.00%,18.00%.Livin protein positive expression rates in colon cancer tissues was more than those in colon adenoma and nomal colorectal mucosa and had statistical significance . (2) The expression of PTEN in colon cancer , colon adenoma and normal colorectal mucosa was 40.00%,76.00%, 98.00%.The expression of Caspase -3 in colon cancer, colon adenoma and normal colorectal mucosa was 46.00%, 84.00%, 92.00%.Caspase-3 and PTEN protein positive expression rates in colon cancer tissues were less than those in colon adenoma and nomal colorectal mucosa (P<0.01).(3) Caspase-3,PTEN protein expression had prominent inverse correlation with the Livin expression .Conclusion Livin,PTEN,Caspase-3 participate the happen and the development of colon cancer .The suppressed of PTEN protein may accelerate the express of Livin , and promote the happening of colon cancer.Livin promotes colon cancer through inhibited caspase -3 protein activity .%目的：探讨Livin基因在结直肠癌中的表达及其与PTEN和Caspase-3的相关性。方法收集结直肠癌、腺瘤及大肠正常黏膜组织各50例，应用免疫组织化学SP染色方法检测Livin、PTEN及Caspase-3的表达情况并进行评分。结果结直肠癌、腺瘤及大肠正常黏膜组织中Livin蛋白的阳性表达率分别为64．00％、56．00％、18．00％，结直肠癌组织中Livin蛋白的阳性表达率高于腺瘤及大肠正常黏膜组织；Livin蛋白阳性表达率与淋巴结转移及Duke分期有关，而与结直肠癌患者的年龄、性别、分化程度无关。在结直肠癌、腺瘤及大肠正常黏膜组织中PTEN蛋白的阳性表达率分别为40．00％、76．00％、98．00％， Caspase -3蛋白的阳性表达率分别为46．00％、84．00％、92．00％，结直肠癌组织中PTEN、Caspase-3蛋白的阳性表达率明显低于腺瘤及正常黏膜组织，差异有显著性意义（P＜0．01）；PTEN、Caspase-3阳性表达率与结直肠癌Duke分期及淋巴结转移密切相关，与结直肠癌患者的年龄、性别及肿瘤分化程度无关。在结直肠癌组织中Livin蛋白阳性表达与PTEN及Caspase-3蛋白表达均呈负相关。结论凋亡抑制因子Livin、凋亡因子Caspase-3及抑癌基因PTEN参与了结直肠癌的发生、发展。在结直肠癌中PTEN蛋白活性被抑制加速了Livin促进结直肠癌的发生。 Livin 促进结直肠癌的发生可能通过抑制Caspase-3的活性实现。