乳腺癌中Wnt信号通路成分NKD1、β-catenin和Cyclin D1蛋白的表达及意义

摘要

Objective To investigate the clinical significance of NKDl,β-catenin,Cyclin D1 protein expression in breast carcinoma.Methods Thirty normal breast tissues and 124 non-specific invasive breast cancer tissues were collected from Dalian Municipal Woman and Children's Medical Center and Dalian Friendship Hospital.Immunohistochemistry was used to detect the expressions of NKD1,β-catenin,Cyclin D1 protein.Spssl7.0 software was used for statistical analysis.Results In breast cancer tissues,the negative frequency of NKD1 expression was 69.35％,the abnormal expression frequency of β-catenin was 72.58％,and the positive frequency of Cyclin D1 expression was 54.03％.There were significant differences between normal breast tissures and breast cancer tissues.Down-regulation of NKD1 expression and abnormal expression of β-catenin were significantly correlated with histologic grades,pTNM stages and the lymph node metastasis of breast cancer (P ＜ 0.05).Up-regulation of Cyclin D1 was significantly correlated with pTNM stages and the lymph node metastasis of breast cancer (P ＜ 0.05).NKD1 expression was positively correlated with β-catenin abnormal expression and Cyclin D1 expression (r =0.454,r =0.332,P ＜ 0.05).Conclusions NKD1,β-catenin,Cyclin D1 protein could become indicators of prognosis in breast cancer.%目的 探讨NKD1、β-catenin和Cyclin D1在乳腺癌中的表达及意义.方法 选取2010年至2016年大连市妇女儿童医疗中心和大连市友谊医院病理科30例正常乳腺组织和124例乳腺浸润性癌非特殊型标本及临床资料.采用免疫组化法检测标本中NKD1、β-catenin和Cyclin D1蛋白的表达,并分析其与临床病理参数之间的关系.结果 在乳腺癌组织中NKD1阳性表达率为30.65％;β-catenin异常表达率为72.58％;Cyclin D1阳性表达率为54.03％,与正常乳腺组织比较,差异均有显著性意义(P均＜0.05).NKD1和β-catenin表达与乳腺癌组织学分级、pTNM分期及淋巴结转移有关,Cyclin D1表达与乳腺癌pTNM分期及淋巴结转移亦有关,且NKD1表达缺失还与ER状态相关,差异均有统计学意义(P均＜0.05).NKD1与β-catenin和Cyclin D1表达呈正相关(r分剐为0.454,0.332,P均＜0.05).结论 NKD1、β-catenin和Cyclin D1蛋白表达与乳腺癌侵袭和转移密切相关,联合检测3种蛋白有助于判断乳腺癌生物学行为和评估预后.