目的 研究基因芯片技术在煤工尘肺合并结核诊断中的应用.方法 所有病人均留取合格痰液,通过基因芯片技术做分枝杆菌菌种鉴定,如为结核分枝杆菌,则做耐药基因检测.结果 结果 显示,结核分枝杆菌阳性病人共15人,阳性率为60%,非结核分枝杆菌病人共10人,阳性率为40%,结核分枝杆菌中有1例对异烟肼耐药,耐药率为6.7%.在10例非结核分枝杆菌中,胞内分枝杆菌6例,占60%,结核分枝杆菌复合群1例,占10%,龟脓或脓肿分枝杆菌1例,占10%.结论 胞内分枝杆菌为非结核分枝杆菌主要致病菌种;基因芯片技术具有简便、快速等特点,可准确区分结核分枝杆菌、非结核分枝杆菌,并可鉴定至种或群,可做耐药监测,可为临床诊断、治疗提供有力的支持.%Objective To explore the diagnostic value of gene clip for coal workers' pneumoconiosis compli-cated with pulmonary tuberculosis. Methods All the patients were used to detect Mycobacterium tuberculosis by gene clip. Mycobacterium strains isolated from all the patients were identified by drug-resistant genes. Results 15 strains of Mycobaterium isolated were M. tuberculosis, accounting for 60%. 10 strains were non-tuberculosis Mycobe-rium, accounting for 40%. In the 15 strains of M. tuberculosis, 1 strain was resistant to isoniazid (6. 7%). 6 strains were M. intracellulare of all the non-tuberculosis Mycobacterium ( 60%) . 1 strain was M. tuberculosis complex group, accounting for 10%. 1 strain was M. chelonae (10%). 2 strains were non-tuberculosis Mycobacterium, but they couldn' t be identified. Conclusion Of all the coal workers' pneumoconiosis complicated with non-tuberculosis Mycobacterium, the main strain is M. Intracellulare. The gene clip is simple, rapid, sensitive and specific for detec-ting tuberculosis. It could diagnose M. tuberculosis and non-tuberculosis Mycoberium and monitor drug resistance, which could be taken as a helpful method for diagnosis and treatment of pulmonary tuberculosis.
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