RNAi干扰脑胶质瘤CEP55表达对胶质瘤细胞LN229功能影响的相关性研究

摘要

Objective To investigate the different expression of centrosomal protein 55(CEP55) in glioma tissues and normal brain tissues,and its effect on proliferation and tumor invasion in glioma cell line LN229 by knocking down the expression of CEP55.Methods 25 cases of glioma tissues and 10 cases of normal brain tissues were collected from Nanjing Brain Hospital to determine the expression of CEP55 via qRT-PCR and Western Blot.The effects of CEP55 on cell proliferation,migration and invasion were evaluated by cell counting,scratching and Transwell tests after knocking down the expression of CEP55 in LN229 cells by RNA interference.ResultsThe expression of CEP55 in glioma tissues was significantly higher than that in normal brain tissues.The function of LN229 cell was declined after interfering the expression of CEP55.Conclusions CEP55 is highly expressed in glioma and may play an important role in cell proliferation,migration and invasion,which will make it became a new molecular therapy target in the future.%目的 探讨中心体相关蛋白55(CEP55)在胶质瘤组织及正常脑组织中的表达差异,以及通过RNAi技术敲低CEP55表达对胶质瘤细胞系LN229功能的影响.方法 收集南京医科大学附属脑科医院25例胶质瘤手术标本及10例正常脑组织标本,采用实时荧光定量PCR(qRT-PCR)及Western blot检测35例样本中CEP55的表达水平.通过siRNA敲低LN229细胞系中CEP55的表达后,采用细胞计数、划痕及Transwell实验检测其对细胞增殖、迁移及侵袭功能的影响.结果 CEP55在脑胶质瘤组织中的表达量明显高于正常脑组织.干扰CEP55的表达后,胶质瘤细胞LN229的功能明显降低.结论 CEP55在胶质瘤中高表达,并参与影响了胶质瘤细胞系LN229的增殖、迁移、侵袭功能.CEP55今后可能成为胶质瘤治疗的新靶点.