异丙酚预处理对大鼠脑缺血再灌注损伤早期中性粒细胞弹性蛋白酶活性及炎性因子表达的影响

摘要

Objective To investigate the effects of Propofol pretreatment on the activity of neutropil elastase (NE) and expression of inflammatory factors including tumor necrosis factor (TNF)-α,interleukin (IL)-1βfollowing early stage of cerebral ischemia-reperfusion (IR) injury in rats. Methods Fifty healthy male SD rats were randomly divided into normal control (NC) group, IR group, Propofol low-dose (P1 , 50 mg/kg) group, medium-dose (P2, 100 mg/kg) group and high-dose ( P3 , 150 mg/kg) group. Focal cerebral ischemia-reperfusion model was established by reversible middle cerebral artery occlusion with filament. Corresponding dose Propofol were injected intraperitoneally 10 min before cerebral ischemia. After cerebral ischemia 2 h and reperfusion 24 h, the neurological deficit score was assessed. The NE activity and expression of TNF-α, IL-1β in brain tissue were detect by enzyme-linked immunosorbent assay. Results Compared with the IR group, the neurological deficit scores in each Propofol group were significantly decreased ( all P < 0. 01) . Compared with NC group, the NE activity and the expression of TNF-α, IL-1β in IR group and Propofol groups were significantly increased (all P<0. 01). Compared with the IR group, the NE activity and the expression of TNF-α, IL-1β in the Propofol groups were significantly decreased ( all P < 0. 01) . The NE activity and the expression of TNF-α, IL-1β in P2 group were significantly lower than those in P1 and P3 groups. Conclusion Propofol pretreatment on early stage of IR injury in rats has cerebral protection, which can reduce the NE activation and inhibit the over-expression of TNF-α, IL-1β and reduce the inflammatory response in brain tissue . The best effect of Propofol pretreatment is 100 mg/kg.%目的 探讨异丙酚预处理对大鼠脑缺血再灌注(IR)损伤早期中性粒细胞弹性蛋白酶(NE)活性及炎性因子肿瘤坏死因子(TNF)-α和白介素(IL)-1β表达的影响.方法 健康雄性SD大鼠50只,随机分为正常对照组(NC组)、IR组、异丙酚低剂量组(P1组,50 mg/kg)、中剂量组(P2组,100 mg/kg)和高剂量组(P3组,150 mg/kg).采用线栓法建立大鼠大脑中动脉局灶性IR损伤模型.各异丙酚组分别于脑缺血前10min经腹腔注射相应剂量的异丙酚.大鼠脑缺血2h、再灌注24h给予神经功能缺损评分,采用酶联免疫吸附法检测脑组织NE活性及TNF-α和IL-1β的表达.结果 各异丙酚组神经功能缺损评分明显低于IR组(均P＜0.01)；与NC组比较,IR组和各异丙酚组脑组织NE活性及TNF-α、IL-1β表达明显升高(均P＜0.01)；与IR组比较,各异丙酚组NE活性及TNF-α、IL-1β表达明显降低(均P＜0.01)；P2组NE活性及TNF-α、IL-1β表达明显低于P1组和P3组(均P＜0.01).结论 异丙酚预处理可抑制脑组织NE活化和TNF-α、IL-1β过度表达,降低炎性反应,对脑组织IR损伤早期具有保护作用；异丙酚100 mg/kg预处理的作用最佳.