高迁移率族蛋白B1在慢性乙型肝炎患者肝组织中的表达及意义

摘要

目的 了解高迁移率族蛋白B1(HMGB1)在慢性乙型肝炎(CHB)患者肝组织中的表达及其与肝组织损伤程度的关系.方法 经血清学证实为慢性乙型肝炎患者46例.对穿刺的肝组织采用常规HE染色、Masson三色特染及Gordon-Sweet银染法观察肝组织炎症与纤维化程度,用免疫组化方法判断HMGBI在肝组织中的表达.结果 正常肝组织HMGB1不表达或在细胞核内微弱表达,HMGB1随肝脏炎症及纤维化加重而表达逐渐增强(P<0.005).结论 HMGB1与肝组织的炎症及纤维化程度密切相关,可作为慢性乙型肝炎患者炎症及纤维化发展以及治疗随访、预后判断的敏感指标.%Objective To investigate the expression of high mobility group box 1 protein( HMGBI ) in different liver tissue and its relationship with liver injury. Methods 46 liver specimens of chronic hepatitis B (CHB) patients were diagnosed by serology test. The hepatic inflammation and fibrosis degree were tested by HE, Masson and Gordon - Sweet staining. Immtmohistochemical method was used to detect the expression of HMGBI. Results HMGBI didn' t or weakly expressed in normal liver tissue, while strongly expressed along with aggravative hepatic inflammation and fibrosis( P ＜ 0. 005 ). Conclusion HMGB1 expression is positively correlated with hepatic inflammation and fibrosis, which is the sensitive indicator of treatment and prognosis.