结肠癌远处转移小鼠模型血清miR-449表达的实验研究

摘要

Objective To explore the experimental significance of expression level of serum miR - 449 in mouse models with distant me-tastasis of colon cancer. Methods The mouse models with distant metastasis of colon cancer were established and the expression of serum miR -449 in different groups (metastasis group,non - metastasis group and control group)of mice had been detected by quantitative PCR. Results In comparison with normal mice,mice in non - metastasis group did not showed elevated expression level of miR - 449 (P > 0. 05). The level of miR - 449 expression in mice of metastasis group had been increased 20. 3 times than that in mice of non - metastasis group,and the difference was significant (P 0.05);转移小鼠组小鼠的miR-449的表达水平较未转移组升高20.3倍,差异有显著显著性(P<0.01).ROC曲线显示,血清miR-44表达水平评判结肠癌转移的灵敏度91％,特异度为94％.结论 血清miR-449表达水平可为临床判定结肠癌远处转移提供新的实验线索,为临床深入研究结肠癌的诊断和治疗提供新的实验依据.