吗啡对人胃癌MGC-803细胞caspase-3表达的影响

摘要

目的 观察吗啡对人胃癌MGC-803细胞凋亡及caspase-3表达的影响.方法 胃癌MGC 803细胞分为两组:对照组和吗啡组.对照组不加任何药物,吗啡组将吗啡加入细胞培养液中使吗啡终浓度为0.1 μmol/L.孵育24 h后应用流式细胞仪检测细胞凋亡情况,并应用半定量逆转录-聚合酶链反应(RT-PCR)和Western blot技术检测胃癌MGC-803细胞中caspase-3基因mRNA和蛋白的表达情况.结果 吗啡组胃癌细胞凋亡率为(17.20±2.95)％,明显高于对照组的(11.40±2.86)％(P＜0.05).RT-PCR和Western blot结果显示,吗啡组胃癌MGC-803细胞中caspase-3mRNA和蛋白表达增加.结论 0.1μmol/L吗啡通过增加caspase-3的表达而促进胃癌细胞的凋亡.%Objective To explore the effects of morphine on the apoptosis of human gastric carcinoma MGC-803 cells and the expression of caspase-3. Methods MGC-803 cells were divided into two groups. The cells were incubated with or without 0. 1 祄ol/L morphine. The apoptosis was assessed by flow cytometry after 24 h. The expression of caspase-3 mRNA and proteins were detected by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot. Results The apoptotic rate of MGC-803 cells in the morphine group was higher than that in the control group. Caspase-3 mRNA and protein were up-regulated by morphine treatment. Conclusion 0.1 祄ol/L morphine may promote the apoptosis of gastric cancer cells by up-regulating caspase-3 in vitro.