Four coumaric derivatives including 3-aminocoumarin, 3-hydroxycoumarin, 3-carhoxycoumarin and 4-methyl-7-hydroxycoumarin were tested as matrices for peptides and proteins analyses by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS). The experimental results showed that all these coumaric derivatives (dissolved in acetonitrile at the concentration of 0. 1mol/L) can desorb/ionize the peptide and protein samples effectively and high resolution MALDI-TOFMS spectra were obtained.
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