玉米须多糖的提取工艺优化及抗氧化活性研究

摘要

研究了玉米须多糖的提取工艺条件和抗氧化活性.通过单因素试验和正交试验确定了最佳工艺参数,即超声处理次数350 ～450次,超声波功率300 W,浸提时间在5～6h,乙醇体积分数约60％.并按200、400 mg/kg给老年大鼠连续灌胃30 d,测血清MDA含量,脑和肝组织脂褐质(Lf)含量,皮肤和肝组织羟脯氨酸(HYP)含量及血清SOD,CAT,GSH - Px活性.结果表明:两剂量组均能明显降低老年大鼠血清MDA含量及脑和肝组织Lf含量,明显提高血清SOD,CAT,GSH - Px活性及皮肤HYP含量.%In this paper, the optimized extraction of corn silk polysaccharide and antiaging action was studied, and the best technological parameter was determined through the single factor experiment and the orthogonal test, namely, supersonic processing number of times was 350 -450, ultrasonic wave power was 300W, the proposed soa- king time was 5 -6h, and ethyl alcohol density was approximately 60%. 200 and 400mg/kg were given to the old rats every day for thirty days. The content of malon -dialdehyde (MDA) in serum, the contents of lipofuscin( Lf) in brain and liver, the content of hydrooxyproline (Hyp) in skin and liver and the activity of superoxide dismutase (SOD) , catalase (CAT) and glutathione peroxidase (GSH -Px) in serum were examined. The results showed that polysaccharide could significantly decrease the content of MDA in serum and the Lf content in brain and liver, but obviously increase the activity of SOD, CAT, GSH - Px in serum and the content of Hyp in skin of the old rats.