LncRNA MEG3对口腔鳞状细胞癌细胞增殖、侵袭及凋亡的影响

摘要

目的 探究LncRNA MEG3在口腔鳞状细胞癌 (oral squamous cell carcinoma, OSCC) 组织和细胞中的表达水平及对OSCC细胞增殖、侵袭和凋亡的影响.方法 qRT-PCR法检测OSCC患者口腔粘膜组织、肿瘤组织及Tca8113细胞系中LncRNA MEG3的表达水平.构建pcDNA3.1-MEG3重组质粒转染Tca8113细胞, 分为3组:转染过表达pcDNA3.1-MEG3的质粒组 (MEG3组) 、空质粒组pcDNA3.1-NC (NC组) 和空白对照组 (BLANK组) .分别采用qRT-PCR法、CCK-8法、流式细胞术和Transwell检测LncRNA MEG3表达、细胞增殖、凋亡率和侵袭能力.结果 与正常口腔黏膜组织和细胞相比, LncRNA MEG3在OSCC组织和细胞中的表达下调 (P<0.05) .体外实验发现, 与空白对照组和NC组相比, 过表达LncRNA MEG3组细胞增殖和侵袭能力受到抑制, 凋亡率明显升高 (P<0.05) .结论 LncRNA MEG3在OSCC组织和细胞中低表达;LncRNA MEG3过表达可抑制OCSS细胞的增殖和侵袭, 促进凋亡.%Objective To investigate the expression of LncRNA MEG3 in the tissues and cells of oral squamous cell carcinoma (OSCC) and its effect on the proliferation, invasion and apoptosis of OSCC cells.Methods The expression of LncRNA MEG3 in the oral mucosa, tumor tissues and Tca8113 cell line of OSCC patients was detected by qRT-PCR.The recombinant plasmid pcDNA3.1-MEG3 was constructed to transfect the Tca8113 cells.The Tca8113 cells transfected with overexpressed pcDNA3.1-MEG3 were divided into the MEG3 group, those transfected with pcDNA3.1-NC were divided into the NC group, and those without any transfection were divided into the blank control group.The expression, cell proliferation, apoptosis rate and invasion ability of LncRNA MEG3 were detected by the methods of qRT-PCR, CCK-8, flow cytometry and Transwell, respectively.Results The expression of LncRNA MEG3 was significantly down-regulated in the OSCC tissues and Tca8113 cells when compared with the normal oral mucosa tissues and cells (P<0.05).The results of vitro experiments showed that the proliferation and invasion ability of the MEG3 group was significantly inhibited and the apoptosis rate was significantly increased when compared with the blank control group and the NC group (P<0.05).Conclusion The expression of LncRNA MEG3 is low in the OSCC tissues and Tca8113 cells, and its overexpression could inhibit the proliferation and invasion of OCSS cells and promote their apoptosis.