Enzymatic detection of ethanol based on H202-sensitive quantum dots

摘要

A rapid and sensitive fluorometric method for the enzymatic detection of ethanol using CdSe/ZnS quantum dots (QDs) is proposed.The photoluminescence of QDs is sensitive to H2O2.This finding leads to a novel approach for the determination of ethanol using alcohol oxidase (AOx) which,on oxidation of ethanol,produces H2O2.The method has higher sensitivity,wider analytical range (0.1-8 mmol/L),and a lower detection limit (0.05 mmol/L).The relationship between quenching of the photoluminescence of the QDs and the concentration of ethanol is linear.