Bioflotation represents one of the growing trends to enhance the selectivity of conventional flotation processes.It utilizes the micro-organisms to replace or to interact with the chemical reagents to increase the gap between surface properties of similar minerals and to enhance the separation selectivity.In this work,dolomite-phosphate separation was investigated using amphoteric collector (dodecyl-N-carboxyethyl-N-hyroxyethyl-imidazoline) in presence of bacteria.Two types of bacteria,Corynebacteriumdiphtheriae-intermedius (CDI),and Pseudomonas aeruginosa (PA),were used.The collector bacteria interaction was characterized by Fourier transform infra-red (FTIR),frothing height and Zeta potential.The results show that the collector bacteria interaction improves the flotation selectivity.Although,the PA positively affects the separation results,the CDI cannot lower the MgO to less than 1%.A phosphate content of 0.7% MgO and 31.77% P2O5 with a recovery of 68% at pH 11,3.0 kg/t amphoteric collector,4×107 cells of PA is obtained.
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