Objective To observe the photoprotective effect and possible mechanisms of resveratrol for ultraviolet A (UVA) irradiated HaCaT cells. Methods HaCaT cells under UVA irradiation with 5 J/cm2 were interfered with 0.01 mmol/L and 0.1 mmol/L resveratrol. The testing objects were divided into a control and a UVA irradiation group, and then we detected the proliferation capacity with methylthiazdyl tetrazolium (MTT) and superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activity, content of maleic dialdehyde (MDA) with hydroxylamine, colorimetric, thiobarbituric acid (TBA) methods. The ultrastructure was observed under electron microscope. Results Resveratrol could enhance the proliferation activity, SOD, GSH-Px activity of HaCaT cells under UVA irradiation, decrease the content of MDA in dose-dependent manner (P＜0.05). The electron microscope revealed that resveratrol could relieve the injury of HaCaT cells'ultrastructure. Conclusion Resveratrol can relieve the inhibition to HaCaT cell proliferation, injury of their ultrastructure and oxidation by UVA irradiation. The protection is dose-dependent. That resveratrol raises the oxidase activity and clears the oxyradical may account for these results.%目的:探讨白藜芦醇对UVA照射后HaCaT细胞的光保护作用及其可能机制.方法:采用5J/cm2 UVA照射HaCaT细胞后,分别加入0.01mmol/L和0.1mmol/L的白藜芦醇进行干预,同时设正常对照组与UVA照射组,分别用MTT法检测细胞增殖能力,用羟胺法、比色法、TBA法检测细胞超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性及丙二醛(MDA)含量,电镜观察细胞超微结构的改变.结果:白藜芦醇能提高UVA照射后HaCaT细胞增殖能力、细胞SOD和GSH-Px活性,降低MDA含量,且呈剂量依赖性增加或降低(P＜0.05).电镜结果显示白藜芦醇能减轻UVA对HaCaT细胞超微结构的损伤.结论:白藜芦醇能减轻UVA对HaCaT细胞增殖的抑制作用、超微结构和氧化功能的损伤,并有随剂量依赖性增加的保护作用,其机制可能与白藜芦醇提高氧化酶活性、清除自由基有关.
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