乳腺癌组织中Livin和Smac的表达及其临床意义

摘要

Objective: To explore the expression and clinic-pathological characteristics of Livin and Smac in breast carci-noma tissue. Methods: S-P immunohistochemical staining was adopted to examine the expression of Livin and Smac; breast cancer samples (n=43) were assembled from West China Hospital, Sichuan University. Results: The positive expression of Livin in breast cancer tissue was 79. 1% , and it was 48. 8% in paracarcinoma tissue. The difference between the two groups was statistically significant (P<0. 05). meanwhile, the positive expression of Smac in breast cancer tissue was 74. 4% , and it was 93. 0% in paracarcinoma tissue. The difference between the two groups was statistically significant (P<0. 05). Livin protein expression in breast cancer was closely related to clinical stage and lymph node metastasis ( P<0. 05). A negative correlation was found between the expression of Livin and that of Samc in breast cancer (P<0. 05). The 5-year disease free survival (DFS) of the Livin-negative group (66. 7% ) was significantly longer than that of the Livin-positive group (23. 5% , P<0. 05), while the 5-year DFS of the Smac-negative group and that of the Smac-positive group were not significantly different ( P >0. 05). Conclusion: The expression level of Livin in breast cancer tissue is negatively related to the expres- sion level of Smac in breast cancer tissue, indicating a negative correlation between these two proteins. meanwhile, the 5 year DFS of Livin-negative patients is significantly longer than that of the Livin-positive patients. Therefore, Livin may be employed as a prognosis indicator for breast cancer patients.%目的:探讨Livin和Smac蛋白在乳腺癌组织和癌旁组织中的表达及其临床意义.方法: 收集乳腺癌患者43例,采用免疫组织化学S-P法检测Livin及Smac在乳腺癌组织、癌旁组织中的表达情况,应用χ2 检验、K-m法分析临床特征及生存情况.结果: Livin在乳腺癌组织中的阳性表达率为79. 1% ,癌旁组织中表达率为48. 8% ,差异有统计学意义(P<0. 05);Smac在乳腺癌组织中的阳性表达率为74. 4% ,癌旁组织中表达率为93. 0% ,差异也有统计学意义(P<0. 05). Livin和Smac蛋白的表达均与乳腺癌的TNm分期及淋巴结转移情况相关,其差异有统计学意义(两组P<0. 05),并且乳腺癌组织中Livin与Smac的表达情况呈负相关(P<0. 05).此外,Livin阴性组与阳性组中5年无病生存率分别为66. 7%和23. 5% ,Livin阴性组比阳性组无病生存期有所延长(P<0. 05),而Smac阴性组与阳性组的无病生存期差异无统计学意义(P>0. 05).结论: Livin、Smac在乳腺癌中的表达水平呈负相关,提示可能有某种机制在调节这两者的表达.另外,Livin阴性组患者的5年无病生存期有所延长,提示该蛋白或可作为乳腺癌患者的预后指标,值得进一步研究.