玉米蛋白3－D 多孔支架材料仿生矿化的初步研究

摘要

In order to promote the application in bone tissue engineering, the zein scaffold was soaked in a solution of 5 times the concentration of simulated body fluid (5 ×SBF) to enhance its bone inductivity and osseointegration.The field emission scanning elec-tron microscopy (SEM) was employed to observe the surface microstructures of the coating and energy dispersive spectrometer (EDS) which was attached to the SEM was used to carry out the elemental analysis and to calculate the ratio of calcium to phosphorus ( Ca/P) .The surface of the composite materials form uniform micro particles(2-10 um) that the ratio of calcium to phosphorus closed to that of hydroxyapatite (HA) after soaked in 5SBF.The zein-HA composite can be considered as an ideal material in bone tissue engi-neering.%为了提高玉米蛋白的骨诱导性和骨结合性，以促进其在骨组织工程领域中的应用，将玉米蛋白多孔支架材料浸泡于5倍模拟体液（5×SBF）中，尝试在不同的条件下对玉米蛋白多孔支架材料表面进行仿生矿化。通过场发射扫描电镜（ SEM）观察仿生矿化后多孔支架材料各个表面的形貌，能谱（ EDS）计算出钙磷比（ Ca/P）比。在5倍模拟体液中浸泡后，材料各表面均形成了分布和尺寸相对均匀的微米级（2～10 um）颗粒。其钙磷比接近羟基磷灰石，可以认为获得了比较理想的玉米蛋白－羟基磷灰石多孔支架复合材料。