PTTG 基因对非霍奇金淋巴瘤细胞增殖能力的影响

摘要

Objective To explore the effects of PTTG gene expression on the reproductive ability of non-hodgkin lymphoma cells SUDHL-4 .Methods Non-Hodgkin Lymphoma cells SUDHL-4 was chose to show the biology behavior changes after si-lence PTTG by shRNA ,through the MTT test ,and cell cycle analysis .Results After silence PTTG by shRNA ,the prolifera-tion of SUDHL-4 cells were significantly decreased ,the ratio of G0/G1 and S phase cells was decreased ,the ratio of G2/M cells was increased ,and cell cycle arrested at G2/M ,the difference was statistically significant (P<0.05) .Conclusion The overex-pression of PT TG gene may be related to the occurrence and development of lymphoma ,and it may become the molecular diag-nostics and new therapy target of lymphoma .%目的：观察垂体瘤转化基因（pituitary tumor-transforming gene ，PTTG）对非霍奇金淋巴瘤细胞SUDHL-4增殖能力的影响。方法通过shRNA的方法在高表达PTTG的SUDHL-4细胞中敲降PTTG基因，通过MTT实验、细胞周期分析检测SUDHL-4生物学行为的变化。结果 PTTG被敲降后SUDHL-4细胞的生长速度明显下降，细胞G0／G1比例、S期比例下降；G2／M期比例升高，细胞阻滞于G2／M期，差异均有统计学意义（P＜0.05）。结论 PTTG基因可能在非霍奇金淋巴瘤的发生发展中起重要作用，可能成为判断非霍奇金淋巴瘤预后的分子标志物及临床治疗的靶点。