目的 研究PTTG基因沉默对胰腺癌细胞增殖的影响.方法 利用阳离子脂质体进行基因沉默实验,将PANC-1细胞分为三组(①.未转染PANC-1细胞、②.转染阴性对照siRNA、③转染siRNA-PTTG),MTT比色法和3H –胸腺嘧啶核苷(3H-TdR)掺入法检测细胞增殖;流式细胞仪分析细胞周期.结果 24 h、48 h、72 h和96 h组③吸光度明显低于与组②和组①,差异具有统计学意义(P＜0.05);组③3H-TdR 掺入率明显低于组②和组①,差异具有统计学意义(P＜0.01),细胞DNA合成抑制率为37.87%;组③G0/G1期细胞百分率显著增加,S期显著降低,差异具有统计学意义(P＜0.05).结论 siRNA-PTTG转染后PANC-1细胞增殖显著抑制,细胞周期阻抑在G0/G1期,DNA合成降低.%Objective To investigate the effect of small interference RNA(siRNA) targeting PTTG gene on the proliferation of pancreatic cancer cell line Panc-1. Methods The chemically synthesized PTTGsiRNA was transfected into cultured Panc-1 cell in vitro by cationic liposomc Lipofcctaminc. PANC-1 cells were divided into three groups as following:①untreated cell ②negative control siRNA ③siRNA-PTTG transfection. Cell proliferation was measured by MTT and intake of 3 H-TdR. Cell cycle was analyzed by flow cytomctry. Results At 24 h、48 h、72 h and 96 h after transfection, the absorbancc of group③was lower than that of the group①and group②. There was statistic difference between them (P<0. 05) ; incorporation of3 H-TdR in group③ was lower than that of the group① and group②. There was statistic difference between them (P<0. 01). The inhibitory rate of DNA synthesis was 37. 87% ; The percentage of G0/G1 phase in group? Increased dramatically and S phase decreased There was statistic difference between them (P<0. 05). Conclusion Following the silencing of PTTG,cell proliferation was inhibited obviously, cell cycle was inhibited at G0/G1 phase and the synthesis of DNA decreased. It may be case by suppression of DNA synthesis.
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