高效液相色谱法检测蜂花粉中总维生素C

摘要

To obtain an efficient method for determination of total vitamin C（VC） in bee pollen,high-performance liquid chromatography（HPLC） was employed and investigated for its stability,precision and repeatability in qualitative and quantitative analysis of total VC in bee pollen.As the results,total VC content and chromatogram peak area presented good linear correlation.The recoveries were 95.71%～98.60% with RSD 1.05%.The RSD of the stability and precision experiments were 2.12% and 1.1% respectively.Total VC content in rape bee pollen was 36.99 mg/100 g in average,and its RSD was 1.43%.In conclusion,HPLC can be used as a simple and fast method for qualitative and quantitative analysis of total VC in bee pollen.%为了得到一种效果较好的测定蜂花粉中总维生素C含量的方法,本文采用高效液相色谱法对油菜蜂花粉进行总VC的定性与定量分析,从结果的稳定性、精密度和重现性三方面对其进行了考量.结果发现,总VC含量在12.70～200.00μg范围内与色谱峰面积呈良好的线性关系,且回收率在95.71%～98.60%,相对标准偏差为1.05%;稳定性实验的相对标准偏差为2.12%,精密度实验的相对标准偏差为1.1%;最终测得油菜蜂花粉样品的总VC平均含量为36.99 mg.（100g）-1,相对标准偏差为1.43%.本研究所建立的HPLC测定蜂花粉中总VC含量的方法简单快速、重现性好,可以较为准确的实现定性与定量分析.