白花蛇舌草对人肝癌 HepG2细胞裸鼠皮下移植瘤 PI3K/Akt 信号通路的影响

摘要

Objective To evaluate the effect of hedyotis diffusa willd on phosphoinositide 3 kinase(PI3K),pro-tein kinase B(Akt)protein and phosphorylated Akt(p-Akt)protein expressions in nude mice with hepatic carci-noma transplanted subcutaneously. Methods Nude mice xenografts were established. The mice were randomly divid-ed into 2 groups:the hedyotis diffusa willd group and the vehicle group,which received hedyotis diffusa willd(6 g·kg -1 ·d -1 ,intragastric administration)and normal saline( intragastric administration),respectively. After 4 weeks of treatment,the tumors were harvested and weighed. The levels of PI3K,Akt and p-Akt proteins were ob-served with immunohistochemical staining. Results The tumor weight of the hedyotis diffusa willd group were high-er than that of the vehicle group(t = 4. 270,P = 0. 006). The gray levels of PI3K,Akt and p-Akt in the hedyotis dif-fusa willd group(118. 67 ± 8. 44,105. 19 ± 4. 718,96. 34 ± 5. 72)were also higher than those in the vehicle group (82. 00 ± 2. 72,72. 37 ± 2. 89,87. 99 ± 4. 16)(t = 0. 209,P = 0. 049;t = 2. 050,P < 0. 001;t = 4. 123,P =0. 002). The expressions of PI3K,Akt and p-Akt proteins were suppressed significantly by hedyotis diffusa willd. Conclusion Hedyotis diffusa willd could inhibit angiogenesis in hepatocellular carcinoma via regulating the PI3K/ Akt pathway.%目的：探讨白花蛇舌草对人肝癌 HepG2细胞裸鼠皮下移植瘤组织磷酸酰肌醇3激酶（PI3K）、总蛋白激酶 B（Akt）和磷酸化 Akt（p-Akt）蛋白表达的影响。方法建立肝癌裸鼠皮下移植瘤模型，随机分为药物组（6 g·kg -1·d -1白花蛇舌草灌胃）和模型组（同等体积生理盐水灌胃），连续给药4周后处死，观察抑瘤率；免疫组织化学染色检测肿瘤组织 PI3K、总 Akt 和 p-Akt 蛋白的表达。结果与模型组比较，药物组瘤体质量明显下降（t =4.270，P =0.006），抑瘤率达61.29%；PI3K 蛋白表达灰度值从82.00±2.72升高至118.67±8.44（t =0.209，P =0.049）；Akt 蛋白表达灰度值从72.37±2.89升高至105.19±4.718（t =2.050， P <0.001），p-Akt 蛋白表达灰度值从87.99±4.16升高至96.34±5.72（t =4.123，P =0.002），提示 PI3K、Akt 和 p-Akt 的蛋白表达水平均较模型组明显减低。结论白花蛇舌草通过可调控 PI3K/ Akt 信号通路的相关蛋白抑制肿瘤血管新生。