惠楼山药的组织培养与快繁技术研究

摘要

[目的]研究惠楼山药组织培养与快繁技术.[方法] 以惠楼山药茎尖部分作为外植体,以MS为基本培养基,添加不同浓度的6-BA 、NAA、IAA、2,4-D、IBA,配制不同的培养基,对惠楼山药试管苗进行分化与诱导、继代与增殖及生根培养试验,并对试管苗进行炼苗与移栽,还提出脱毒快繁后的惠楼山药的栽培要点.[结果] 经分化与诱导培养后的无毒茎尖分化苗接种到继代与增殖培养基中,5 d后有愈伤组织形成,28～35 d苗高3～5 cm,将生长势强、健壮的苗转入生根培养基中,25 d左右生根率达95%以上,生根28～35 d的幼苗经炼苗和移栽后,成活率在90%以上.[结论]获得了惠楼山药茎尖培养脱毒与诱导、增殖和生根的培养基,以及生长所需要的外部环境条件和配套技术.%[Objective]The study aimed to study the virus-free tissue culture and the rapid propagation technology.[Method] With yam shoot tip as explants,MS was used as the basic medium with different concn.of 6-BA,NAA,IAA,2,4-D and IBA to configure different media,the tests of differentiation and induction,subculture and proliferation as well as rooting culture on Huilou yam were carried out and the test-tube plantlets were hardened and transplanted.The key cultivation techniques for Huilou yam after virus-free rapid propagation were also put forward.[Result] When non-toxic differentiated seedlings of shoot tip after differentiation and induction culture were inoculated on subculture and proliferation medium,callus formed after 5 d,and seedling height was 3-5 cm after 28-35 d.The strong seedlings were transferred into the rooting medium,and then they got the rooting rate of over 95% after about 25 d.Finally the seedlings that rooted for 28～25 d had survival rate of over 90% after hardening and transplanting.[Conclusion] In the experiment,the media of detoxification and induction,proliferation and rooting were obtained as well as the external environmental conditions for the plant growth and the matching technology.