芹菜叶中总黄酮的提取工艺研究

摘要

[Objective] To investigate the optimum extraction technology of total flavonoids from celery leaves by using Soxhlet extraction method, so as to provide a theoretical basis for the development and utilization of celery leaves. [ Method] With the ethanol as the solvent,Soxhlet method was adopted to extract total flavonoids from celery leaves. Effects of ethanol concentration,extraction time,extraction temperature and solid-liquid ratio on the yield of total flavonoids were res|)ectively investigated through the single-factor test. [Result] Factors that affected the yield of total flavonoids were in the order of ethanol concentration > solid-liquid ratio > extraction temperature > extraction time. The optimum technology conditions of total flavonoids from celery leaves by using Soxhlet extraction method were us follows:ethanol concentration of 60% ,extraction time of 1 h .extraction temperature of 70 ℃ and solid-liquid ratio of 1: 60. Under the optimum condition, the yield of total flavonoids was 47.85 mg/g. [Conclusion] The Soxhlet extraction method was available for extracting total flavonoids from celery leaves.%[目的]探讨从芹菜叶黄酮类成分的最佳工艺,为芹菜叶的开发利用提供理论依据[方法]以乙醇为提取溶剂,采用索式抽提法从芹菜叶中提取黄酮类物质成分,通过单因素试验研究乙醇浓度、提取时间、提取温度和料波比对总黄酮得率的影响.通过正交试验优选最佳提取工艺.[结果]影响黄酮得率的因素主次顺序为:乙醇农度＞料液比＞提取温度＞提取时间.芹菜叶中总黄酮的最佳提取工艺为:提取溶剂乙醇的浓度为60％,提取时间为1 h,提取温度为70℃,料液比为1∶60.在此工艺条件下,提取的总黄酮得率为47.85mg/g.[结论]索式抽提法提取芹菜叶中的黄酮是一种切实可行的方法.