[目的]对红曲霉JF-02液体发酵产红曲色素的发酵工艺进行优化.[方法]利用单因素试验考察了温度、起始pH、培养时间、不同农副产品、氮源对发酵液中红曲霉色素含量的影响,同时利用正交试验得到最适的培养基和培养条件.[结果]红曲霉菌种产色素最优培养基组成为大米粉200 g/L、番薯粉30 g/L、葡萄糖10 g/L、谷氨酸钠15 g/L、硫酸锌0.1％,硫酸镁0.1％；最优培养条件为温度30℃,起始pH 6.0,发酵时间72h.但是24-L发酵罐培养时,培养时间延长到84h.[结论]该研究可为红曲色素的开发应用提供理论依据.%[Objective] The aim was to study the fermentation technology of monascus pigment of monascus strains JF-02. [Method] Single factor experiment was carried out to study the influence of temperature, initial pH, culture time, different agricultural byproduct, and nitrogen source on monascus pigment in fermentation solution. Meanwhile, orthogonal experiment was conducted to get the optimal culture medium and cultivation condition. [ Result] The optimal gene in the pigment of monascus pigment was 200 g/L of rice, 30 g/L of sweet potato powder, 10 g/L of glucose, 15 g/L of monosodium glutamate, 0. 1% of zinc sulfate, and 0. 1% of magnesium sulfate. The optimal culture condition was 30℃ and initial pH was 6.0. Fermentation time was 72 hf but when 24-L fermentation pot was used, culture time can last to 84 h. [ Conclusion] The study provided theoretical basis for the development and application of monascus strains.
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