[目的]研究抗菌药物恩诺沙星、环丙沙星、加替沙星对27株副猪嗜血杆菌临床分离株体外抗菌活性,从而观察副猪嗜血杆菌对喹诺酮类抗菌药物的敏感性.[方法]采用微量肉汤稀释法测定MIC,参考标准为美国临床和实验室标准协会(CLSI).[结果]恩诺沙星(MIC50,MIC90,0.25μg/mL,2μg/mL)、环丙沙星(MIC50,MIC90,0.125μg/mL,2μg/mL)、加替沙星(MIC50,MIC90,0.125μg/mL,1μg/mL)均表现出一定的抑菌活性,且加替沙星的抑菌活性最明显.在耐药性方面,副猪嗜血杆菌对恩诺沙星的耐药性最强(22.2%),环丙沙星(3.8%)和加替沙星(7.4%)具有轻微的耐药性.[结论]抗菌药物恩诺沙星、加替沙星以及环丙沙星对副猪嗜血杆菌临床分离株具有一定的抗菌活性,在耐药性方面,HPS已经对恩诺沙星表现出一定的耐药性,因此,今后在临床上遇到副猪嗜血杆菌引起的相关疾病,可以采用合理的给药方案进行疾病的治疗,对于预防耐药菌株产生至关重要.%[Objective ]The purpose of the research is to evaluate the activity of the enrofloxacin,ciprofloxacin,gatifloxacin against heamophilus parasuis(27 isolates) , and we can estimate the ability of the quinolone compounds and oxazolidinone antibacterial against heamophilus parasuis (HPS).[Method] Broth microdilution method was used to determine MIC, following the Clinical and Laboratory Standards Institute (CLSI) rec-ommendations.[Result] Enrofloxacin(MIC50,MIC90,0.25ug/mL,2ug/mL),ciprofloxacin(MIC50,MIC90,0.125ug/mL,2ug/mL) , gatifloxacin (MIC50,MIC90,0.125ug/mL,1ug/mL)exhibited activity against HPS,gatifloxacin was the best one of compounds in aspect of inhibition HPS.In repect of resistance , HPS was most resistant for enrofloxacin(22.2%), and showed a little resistance for ciprofloxacin(3.8%) and gatifloxacin (7.4%) .[Conclusion] The compounds of quinolone revealed better antibacterial activity than the oxazolidinone antibacterial-linezolid, and linezolid dosen' t show the function to restrain HPS.As respect of resistance , HPS reveals severe resistance for enrofloxacin .So , it is important for us to adopt the right ways to choose relevant drugs , when we encounter the diseases caused by HPS.
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