2型糖尿病并发血管疾病男性患者雄激素受体基因(CAG)n多态性研究

摘要

目的 研究雄激素受体(AR)基因(CAG)n多态性分布及其与2型糖尿病并发血管疾病的相关性.方法 采用序列特异性引物PCR(SSP-PCR)和DNA测序技术检测48例2型糖尿病并发血管疾病的男性患者(A组)的AR基因CAG串联重复序列(CAG-STR)长度,并与60例无血管并发症的2型糖尿病男性患者(B组)和46例健康对照组比较.结果 154例研究对象的CAG重复次数介于13～36次,平均22.2次.A、B、健康对照组CAG重复次数分别为(23.4±3.85)、(22.3±3.57)、(21.9±3.45)次,A组与B组、健康对照组之间的差异均有统计学意义(P<0.05).CAG重复次数与HDL-C水平呈正相关(r=0.289,P=0.003).结论 CAG重复序列的长度可能是2型糖尿病并发血管疾病的危险因素,CAG重复序列越长,2型糖尿病患者并发血管疾病的可能性越大.%Objective To study the relationship of androgen receptor (AR) gene (CAG)n polymorphism frequency and angiopathy in male patients of type 2 diabetes mellitus. Methods PCR and DNA sequence methods were used to detect androgen receptor gene CAG short tandem repeat (CAG-STR) repeats length in 48 cases of type 2 diabetes patients with vascular complications (group A) , 60 type 2 diabetes patients without vascular complications (group B),and 46 cases of normal controls (normal control group). Results The numbers of CAG repeats ranged from 13 to 36 , with mean value 22. 2. The numbers of CAG repeats of group A, group B,and normal control group were 23. 4 + 3. 85,22. 3 + 3. 57, and 21. 9 + 3. 45 , respectively. CAG-STR repeats length of group A was significantly higher than that of group B and normal control group(P<0. 01). The numbers of CAG repeats were positively correlated with HDL-C levels. Conclusion Diabetic vascular complications might be associated with androgen receptor CAG gene polymorphism. The number of CAG repeats is likely to be a risk factor of diabetic vascular disease.