桥本甲状腺炎患者外周血CD4＋T 细胞瘦素检测及临床意义

摘要

Objective To detect the level of CD4+ T cell-derived leptin(LP) in peripheral blood mononuclear cells(PBMC) from the patients with Hashimoto′s thyroiditis(HT) and to investigate its role in the occurrence and development of HT .Methods The peripheral blood samples in the patients with HT and the healthy controls were collected and separated for obtaining PBMC .The CD4+ T cells magnetic beads was adopted to separate CD4+ T cells for culturing in vitro .The LP level in supernatant was detected by ELISA .The relative expression amount of the orphan nuclear receptor RORγt was detected by real-time quantitative PCR (qRT-PCR) method .Results The LP level of CD4+ T cell culture liquid in the HT patients was markedly higher than that in the healthy control group ,difference was statistically significant (P<0 .05) .Furthermore ,the LP level in CD4+ T cell culture liquid was posi-tively related with the relative expression amount of RORγt in HT patients .Conclusion The CD4+ T cell-induced leptin level is in-creased in HT patients ,which is closely related with the occurrence and development of HT .%目的：检测桥本甲状腺炎（H T ）患者外周血CD4＋ T细胞瘦素（L P ）表达的水平，探讨其在 H T发生、发展中的作用。方法收集 HT患者和健康对照者外周血标本，分离外周血单核细胞（PBMC），再用CD4＋ T细胞免疫磁珠分离CD4＋ T细胞，体外进行培养，酶联免疫吸附试验（ELISA）检测上清液中LP水平。采用实时定量PCR（qRT-PCR）方法检测PBMC中孤儿核受体γt（RORγt）相对表达量。结果 HT患者CD4＋ T细胞培养液中LP水平明显高于健康对照组，差异有统计学意义（P＜0．05）。HT患者CD4＋ T细胞培养液中LP水平与RORγt相对表达量呈正相关。结论 HT患者CD4＋ T 细胞LP水平升高，与疾病的发生发展关系密切。