细粒棘球蚴 GST 抗原表位的生物信息学预测∗

摘要

Objective To analyse the secondary structure of glutathions S-transferase of Echinoccocus granulosus(EgGST)and predict the B cell and T cell epitopes with informatics tools,in order to provide basic data and references for the following design of epitope vaccine.Methods The B cell and T cell epitopes of EgGST were predicted by DNAstar,Biosun software and Propred MHC class-Ⅱ Binding Peptide Prediction Server software.Results Many distinct antigenic epitopes of EgGST were identified by comput-er,there existed 8 B cell epitopes and 7 T cell epitopes.Conclusion Analysis of predicted epitopes of EgGST antigenic might be sig-nificant for future research of epitope vaccine.%目的：应用生物信息软件分析细粒棘球蚴谷胱甘肽 S-转移酶(EgGST)的蛋白二级结构并预测 B 淋巴细胞表位和 T淋巴细胞表位，为后续表位疫苗的设计研究提供基本的数据和资料。方法采用 DNAstar 软件、ProPred MHC Class-Ⅱ Binding Peptide Prediction Server 和 Biosun 软件对 EgGST 的 B 淋巴细胞表位和 T 淋巴细胞表位进行预测。结果预测 EgGST 存在8个 B 淋巴细胞表位区域和7个 T 淋巴细胞表位。结论分析预测的 EgGST 抗原表位将对后续的表位疫苗研究具有重要意义。